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-  2017 

Structural Properties of Casein Micelles in Milk, the effect of salt, temperature, and pH

DOI: 10.25141/2475-3432-2017-6.0202

Keywords: The radial distribution function (r.d.f or g(r)) is the variation of the number density (e??? = !, the unit of e??? is number/volume) of particle as a function of the ! distance from a certain particle, see schematic picture in Figure 12 (left). Or more simply spoken: it is the probability of finding a particle at a distance r far from a reference particle. shows the radial distribution between charged micelles (blue curve), and neutral micelles (red curve). The radial distribution function versus the distance between the micelles for two different systems. The blue curve corresponds to a system with 21 neutral micelles, while the red curve corresponds to a system with 21 neutral micelles together with 2940 neutral a??counterionsa??. shows the response in the micelle- a??micelle struc - ture with respect to the addition of 80 mM of monovalent salt Correlations effects. Each half of the system is electroneutral, and the blue spheres correspond to salt ions of higher valency, and the surfaces correspond to negatively charged colloids. In (a) the two colloids are well separated and do not feel each other, (b) they feel each other and charge fluctuations are induced, which results (c) in the aggregation of the colloids. shows the difference in the distribution of micelles with the variation of the valency of the counterions. In the green curve, the micelles look freer than in the red and the blue curve, and this behavior occurred because of the screening of the electrostatic force, which increases sharply when increasing the valence. The maxima of the r.d.f:s are varying as well, which indicate the variations of structures. The micellar structure has been studied as an effect of temperature to mimic the pasteurization process. shows the g(r) for micelles in ordinary milk (blue curve) and in fermented milk (red curve) as well as test micelle where the charge is 1/3 of the micellar charge in milk.

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Abstract:

Milk is a complex liquid, which contains many different species, for example proteins, fat, minerals etc. It is the primary source of nutrition for young mammals before they are able to digest other types of food. The proteins can be divided into two groups: caseins and whey. Whey proteins are about 20 wt % of the total protein amount in milk, whereas the caseins corresponds 80 wt % of the total protein content in milk. The largest structures in the fluid portion of the milk are a??casein micellesa?? which are aggregates of several thousands of protein molecules. The micelle is considered to be spherical and the diameter is in the micrometer size

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