THE CYTOTOXIC EFFECT of 5-FU AND CELASTRO L on GLIOBLASTO MA

Glioblastoma is the most malign and agressive brain tumor. Glioblastoma has heterogeneity in terms of genotypically and phenotypically and also there is no precise treatment model. Chemotherapy has a big role in the treatment of glioblastoma but chemotherapeutic resistance decrease the effectiveness of treatment. Anticancerogenic molecules which derived from plants are devoloping with the purpose of decreasing chemotheraupatic resistance and effective treatment. In recent years, as an important treatment approach, the combination of these molecules with chemotherapeutic agents as well as single administration increases. In this study, it was aimed to investigate the cytotoxic effect of the combination of chemotherapeutic agent 5- Fluorouracil and a natural compound Celastrol in glioblastoma. As a result of the MTT cytotoxicity test, it was determined that Celastrol (2μM) has increased the cytotoxic effect of 5- Fluorouracil (50 μM) by 19%, the cytotoxic effect of 5-Fluorouracil (100 μM) has increased by celastrol (0.5 and 2 μM) in ratio of 25% and 37% , respectively. This is the first study to show the effects of 5-Fluorouracil and celastrol combination in all types of cancer. This study, which demonstrated the importance of the use of celastrol with 5-Fluorouracil fo r the first time, may provide a basis for further research in order to develop a new treatment protoco