Investigation of the Differences of Immunological Characteristics of Mecsina Hemostopper？, Ankaferd Blood Stopper？ and Tranexamic Acid Used as Haemostatic Agents with Cell Culture Study

Objective: Hemorrhagic complications may develop in many branches of medicine and dentistry after or during the treatment independently of the extent of the procedure performed. Various biomaterials have been used in the medical and dental practice procedures before and after surgical procedures. The aim of this study was to compare the immunological efficacies and effects of Ankaferd Blood Stopper, Tranexamic acid used as anti-hemorrhagic agents and Mecsina Blood Stopper, a new anti-hemorrhagic agent. Method: The immortalized HUVEC (Human Umbilical Vein Endothelial Cell) cell lines, which are human umbilical cord cells and used in many studies on coagulation and formation of fibrin, were commercially purchased for the study. The cells, 5000 cells per flask for each drug, were distributed into the 5 groups (ankaferd, Tranexamic acid, mecsina, distilled water-administered negative and control without any administration). Results: According to the data we obtained, TNF alpha (Tumor Necrosis Factor Alpha) levels were found to be lower in the cells, to which mecsina was administered at concentrations of 1: 2 and 1:10, than other drug groups (p<0,05). There was a significant increase in IL-1B (Interleukin 1B) and IL6 (Interleukin 6) levels in all drug administrations at both concentrations of 1: 2 and 1: 10 compared to the control group (p <0,05). While there was no significant increase in TNF alpha levels in all drug administrations at a concentration of 1: 2 (p <0,05), there was no significant correlation in mecsina administration at a concentration of 1:10 (p> 0,05), but a significant increase was found in tranexamic acid (TA) and ankaferd administrations (p <0.05). Conclusion: In conclusion, it has been observed that different anti-hemorrhagic agents administered at different concentrations in HUVEC cell groups produced a significant increase in intracellular cytokine levels. Considering the results, we can say that MH (Mecsina Hemostopper) administration is a more effective anti-hemorrhagic agent than administrations of ABS (Ankaferd Blood Stoper) and especially TA (Tranexamic Acid)