Effect of Vitamin D Receptor rs1544410 Polymorphism on Tooth Decays

Due to the effects of vitamin D on muscle and bone development, there are significant effects on oral and dental health. One of the important markers of oral health is the index (DMF-T), which reports the number of caries, missing filled teeth. In our study, the relationship between rs1544410 polymorphism on D vitamine receptor (VDR) and the number of teeth affected by caries and results was investigated. A total of 24 athletes, 11 girls and 13 boys, participated in the study. The DNA samples of the athletes were isolated from buccal cells by using a commercially avaible DNA isolation Kit. The real-time polymerase chain reaction (RTPCR) method was used to determine the genotypes of the athletes. The DMF-T index was used to identify individuals with caries, missing filled teeth. Respective number and percentages of AA, AG, GG genotypes were found as 8 (33%), 13 (54%) and 3 (12%) GG, for rs1544410 polymorphism. When allele distributions were examined, A allelle was found to be as 60%, and G allelle as 40%. When genotypes were compared with DMF-T, 2 athletes were (25%) between 0-1, 4 athletes (50%) between 2-4, 2 athletes (25%) were between 5-10 , and none of the athletes had above 10 for the individuals with AA genotypes. For AG genotypes, the respective numbers and percentages of individuals with values 0-1, 2-4, 5-10, 10 and above are 3 (23%), 3 (23%), 5 (38%) and 2 (16%). For GG genotypes, the DMFT index was found in 2 athletes with a score of 0-1 (67%) and in 1 (33%) with a score of 2-4. None of the athletes with GG gonotypes had 5-10 or 10 and above score for DMF-T. We suggest that this study may be an informative data for further studies to investigate the effect of rs1544410 polymorphisms on the formation of tooth decays