Osprey is a type of bird of prey that lives almost
all over the world. In Japan, it is designated as a near-threatened species
because it has less than 1000 individuals. In recent years, it inhabits more
inland than in coastal areas. In this study, we conducted a population genetic
analysis focusing on what kind of genetic structure Japanese Osprey retains and
whether there are differences between coastal and inland populations. We also
performed genetic diversity assessments. We sequencedabout
2.3 kb of mtDNA for 27 individuals in Japan, and phylogenetic analysis, network
analysis, neutrality test and mismatch distribution analysis were performed. Eighteen
haplotypes were detected in 27 individuals, indicating that genetic diversity
was sufficiently high. Both unique and common haplotypes were detected between
inland and coastal populations, suggesting gene flow between the two
populations. Phylogenetic analysis results show no genetic differentiation in
the Japanese Osprey population. From the results of network analysis,
neutrality test and mismatch distribution analysis, it was inferred that the
Japanese Osprey had a population expansion in the past. This study indicated
that the dispersion of Japanese Osprey was random and there were no
restrictions on the breeding area. The information presented here can be used
towards implementing future conservation actions.
References
[1]
Green, R. (1976) Breeding Behaviour of Ospreys Pandion haliaetus in Scotland. Ibis, 118, 475-490. https://doi.org/10.1111/j.1474-919X.1976.tb03513.x
[2]
Brazil, M. (1991) The Birds of Japan. Christopher Helm, London.
[3]
Brazil, M. (2009) Birds of East Asia. Princeton University Press, Princeton.
[4]
Francour, P. and Thibault, J.C. (1996) The Diet of Breeding Osprey Pandion haliaetus on Corsica: Exploitation of Coastal Marine Environment. Bird Study, 43, 129-133.
https://doi.org/10.1080/00063659609461004
[5]
Shoji, A., Sugiyama, A. and Brazil, M.A. (2011) The Status and Breeding Biology of Ospreys in Hokkaido, Japan. The Condor, 113, 762-767.
https://doi.org/10.1525/cond.2011.110041
[6]
Lerner, H.R.L. and Mindell, D.P. (2005) Phylogeny of Eagles, Old World Vultures, and Other Accipitridae Based on Nuclear and Mitochondrial DNA. Molecular Phylogenetics and Evolution, 37, 327-346. https://doi.org/10.1016/j.ympev.2005.04.010
[7]
Griffiths, C.S., Barrowclough, G.F., Groth, J.G. and Mertz, L.A. (2007) Phylogeny, Diversity, and Classification of the Accipitridae Based on DNA Sequences of the RAG-1 Exon. Journal of Avian Biology, 38, 587-602.
[8]
The Ornithological society of Japan (2012) Check-List of Japanese Birds. 7th Revised Edition, the Ornithological Society of Japan, Sanda.
[9]
Gill, F. and Donsker, D. (2020) IOC World Bird List (v10.2).
[10]
Monti, F., Duriez, O., Arnal, V., Dominici, J.M., Sforzi, A., Fusani, L., Gremillet, D. and Montgelard, C. (2015) Being Cosmopolitan: Evolutionary History and Phylogeography of a Specialized Raptor, the Osprey Pandion haliaetus. BMC Evolutionary Biology, 15, Article No. 255. https://doi.org/10.1186/s12862-015-0535-6
[11]
Ministry of the Environment (2012) How to Proceed with the Protection of Raptors. Japan Ministry of the Environment, Tokyo. (In Japanese)
[12]
Ministry of the Environment (2014) Red Data Book: Threatened Wildlife of Japan. Vol. 2, Aves Gyosei, Tokyo. (in Japanese)
[13]
Morioka, T., Kanouchi, T., Kawata., T. and Yamagata, N. (1995) The Birds of Prey in Japan. Bun-ichi Sogo Shuppan, Tokyo. (In Japanese)
[14]
Poole, A.F. (1989) Ospreys: A Natural and Unnatural History. Cambridge University Press, New York.
[15]
Kawaguchi, S. (2004) Nest Sites of the Osprey Pandion haliaetus in the Eastern Part of Kagawa Prefecture, Japan. Japanese Journal of Ornithology, 53, 36-39.
https://doi.org/10.3838/jjo.53.36
[16]
Yamazaki, T. (2000) Raptor Conservation and Its Relationship to the Biodiversity Conservation of Environment. Landscape Research, 64, 311-314. (in Japanese)
https://doi.org/10.5632/jila.64.310
[17]
Kimura, H., Abe, Y., Yanbe, E. and Ogasawara, K. (2007) Inducement of a Pair of Ospreys Pandion haliaetus to Utilize an Artificial Nest. Journal of the Yamashina Institute for Ornithology, 39, 31-34. (In Japanese) https://doi.org/10.3312/jyio.39.31
[18]
Ministry of Land, Infrastructure, Transport and Tourism (2017) A Review of the Results of the Past Census of River Waterfront. Ministry of Land, Infrastructure, Transport and Tourism, Tokyo. (In Japanese)
[19]
Sakakibara, T., Noguchi, M., Yoshii, C. and Azuma, A. (2020) Diet of the Osprey, Pandion haliaetus, in Inland Japan. Ornithological Science, 19, 81-86.
https://doi.org/10.2326/osj.19.81
[20]
Mori, K., Sakakibara, T., Noguchi, M., Yoshii, C. and Azuma, A. (2020) Food Habits of Ospreys during Brood Rearing in Coastal Areas of Iwate Prefecture, Japan. Bird Research, 16, A15-A24.
[21]
Avise, J.C., Arnold, J., Ball, R.M., Bermingham, E., Lamb, T., Neigel, J.E., et al. (1987) Intraspecific Phylogeography: The Mitochondrial DNA Bridge between Population Genetics and Systematics. Annual Review of Ecology and Systematics, 18, 489-522. https://doi.org/10.1146/annurev.es.18.110187.002421
[22]
Nagata, J., Masuda, R., Kaji, K., Kaneko, M. and Yoshida, M.C. (1998) Genetic Variation and Population Structure of the Japanese Sika Deer (Cervus nippon) in Hokkaido Island Based on Mitochondrial D-Loop Sequences. Molecular Ecology, 7, 871-877.
https://doi.org/10.1046/j.1365-294x.1998.00404.x
[23]
Avise, J.C. (2000) Phylogeography: The History and Formation of Species. Harvard University Press, Cambridge. https://doi.org/10.2307/j.ctv1nzfgj7
[24]
Nagai, K., Takahashi, Y., Tokita, K., Uchida, K., Anzai, T. and Nakayama, F. (2020) Genetic Diversity in Japanese Populations of the Eurasian Collared Dove. Avian Research, 11, Article No. 21. https://doi.org/10.1186/s40657-020-00207-8
[25]
Thompson, J.D., Higgins, D.G. and Gibson, T.J. (1994) CLUSTAL W: Improving the Sensitivity of Progressive Multiple Sequence Alignment through Sequence Weighting, Position-Specific Gap Penalties and Weight Matrix Choice. Nucleic Acids Research, 22, 4673-4680. https://doi.org/10.1093/nar/22.22.4673
[26]
Rozas, J., Ferrer-Mata, A., Sánchez-DelBarrio, J.C., Guirao-Rico, S., Librado, P. and Ramos-Onsins, S.E. (2017) DnaSP 6: DNA Sequence Polymorphism Analysis of Large Datasets. Molecular Biology and Evolution, 34, 3299-3302.
https://doi.org/10.1093/molbev/msx248
[27]
Clement, M.D., Posada, D. and Crandall, K.A. (2000) TCS: A Computer Program to Estimate Gene Genealogies. Molecular Ecology, 9, 1657-1659.
https://doi.org/10.1046/j.1365-294x.2000.01020.x
[28]
Rogers, A.R. and Harpending, H. (1998) Population Growth Waves in the Distribution of Pairwise Genetic Differences. Molecular Biology and Evolution, 9, 552-569.
https://doi.org/10.1093/oxfordjournals.molbev.a040727
[29]
Excoffier, L. and Lischer, H.E.L. (2010) Arlequin Suite Ver 3.5: A New Series of Programs to Perform Population Genetics Analyses under Linux and Windows. Molecular Ecology Resources, 10, 564-567.
https://doi.org/10.1111/j.1755-0998.2010.02847.x
[30]
Tajima, F. (1983) Evolutionary Relationship of DNA Sequences in Finite Populations. Genetics, 105, 437-460. https://doi.org/10.1093/genetics/105.2.437
[31]
Fu, Y.X. (1997) Statistical Tests of Neutrality of Mutations against Population Growth, Hitchhiking and Background Selection. Genetics, 147, 915-925.
https://doi.org/10.1093/genetics/147.2.915
[32]
Harpending, H.C., Sherry, S.T., Rogers, A.R. and Stoneking, M. (1993) The Genetic Structure of Ancient Human Populations. Current Anthropology, 34, 483-496.
https://doi.org/10.1086/204195
[33]
Harpending, H.C. (1994) Signature of Ancient Population Growth in a Low-Resolution Mitochondrial DNA Mismatch Distribution. Human Biology, 66, 591-600.
[34]
Yang, Z. (1994) Maximum Likelihood Phylogenetic Estimation from DNA Sequences with Variable Rates over Sites: Approximate Methods. Journal of Molecular Evolution, 39, 306-314. https://doi.org/10.1007/BF00160154
[35]
Kumar, S., Stecher, G. and Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33, 1870-1874. https://doi.org/10.1093/molbev/msw054
[36]
Asai, S., Yamamoto, Y. and Yamagishi, S. (2006) Genetic Diversity and Extent of Gene Flow in the Endangered Japanese Population of Hodgson’s Hawk-Eagle. Spizaetus nipalensis. Bird Conservation International, 16, 113-129.
https://doi.org/10.1017/S0959270906000050
[37]
Nagai, K., Iseki, F. and Azuma, A. (2019) Analysis of the Genetic Diversity and Structure of the Grey-Faced Buzzard (Butastur indicus) in Japan, Based on mtDNA. Zoological Science, 36, 17-22. https://doi.org/10.2108/zs180077
[38]
Nagai, K., Nakayama, F., Tokita, K. and Kawakami, K. (2019) Genetic Structure and Diversity of Two Populations of the Eastern Buzzard (Buteo japonicus japonicus and B. j. toyoshimai) in Japan. Zoological Science, 36, 471-478.
https://doi.org/10.2108/zs190030
[39]
Nagai, K., Manawatthana, S., Tokita, K., Nualsri, C., Pierce, A.J., Sutasha, K., Sribuarod, K., Takehara, K., Round, P.D. and Higuchi, H. (2020) Genetic Structure in Japanese and Thai Populations of the Japanese Sparrowhawk Accipiter gularis. Zoological Science, 37, 232-239. https://doi.org/10.2108/zs190104
[40]
Nagai, K., Takahashi, Y., Yamazaki, S. and Azuma, A. (2018) Analysis of the Genetic Diversity and Structure of the Eastern Marsh Harrier in Japan Using Mitochondrial DNA. Journal of Ornithology, 159, 73-78.
https://doi.org/10.1007/s10336-017-1480-5
[41]
Yamashina Institute for Ornithology (2011) Report on the Japanese Bird Banding Scheme for 2011. Yamashina Institute for Ornithology, Chiba, 46. (In Japanese)
[42]
Houghton, L.M. and Rymon, L.M. (1997) Nesting Distribution and Population Status of U. S. Ospreys 1994. Journal of Raptor Research, 31, 44-53.
