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暗纹东方鲀与红鳍东方鲀鳔的转录组比较分析
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Abstract:
为了探究暗纹东方鲀和红鳍东方鲀的差异，本研究通过转录组测序技术(RNA-Seq)及生物信息学分析比较了暗纹东方鲀和红鳍东方鲀的鳔组织(TOSB vs TRSB)在转录水平上的差异。结果表明：在两种东方鲀中共得到了4062个差异表达基因(以p.adj < 0.05 & |log2foldchange| ≥ 1为阈值)，其中上调表达的差异基因有2282个，下调表达的差异基因有1780个；通过GO和KEGG富集分析发现，差异基因显著富集到了与信号传导和听力相关的通路中。研究表明两种东方鲀在信号传导和听力功能方面存在显著差异，这种差异可能与其生存环境的不同相关。本研究为暗纹东方鲀与红鳍东方鲀的后续研究提供了数据支持，同时也为其他生物鳔组织的研究提供了参考。
In order to explore the differences between the Takifugu obscurus and Takifugu rubripes, we compared the results of the transcription level in the two fish swim bladders (TOSB vs TRSB) via transcriptome sequencing technology (RNA-Seq) and bioinformatics analysis. In the present study, p.adj < 0.05 & |log2foldchange| ≥ 1 were set as the threshold for screening differentially expressed genes (DEGs). Results showed that a total of 4062 genes were identified as DEGs in the two Oriental pufferfish, including 2282 up-regulated DEGs and 1780 down-regulated DEGs. In addition, GO and KEGG enrichment analysis revealed that the terms or pathways related to signaling and hearing were significantly enriched. Studies have shown that the two puffer fish were significantly different in signal transduction and hearing function, which may be related to differences in their living environments. In conclusion, our study offered data supporting for the follow-up research of T. obscurus and T. rubripes, as well as providing a reference for the study of swim bladder tissues in other fish species.
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