The purpose of this study was to establish a high-performance liquid chromatography (HPLC) method for the simultaneous determination of sodium danshensu, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, and 4-coumaric acid in Danhong injection. The chromatographic method employed was as follows: the column was a Welch Ultimate XB-C18 column (250 mm × 4.6 mm, 10 μm), the mobile phase was a gradient elution of 0.4% formic acid aqueous solution (A) and acetonitrile (B), the detection wavelengths were 280 nm for sodium danshensu, protocatechuic aldehyde, and salvianolic acid B and 326 nm for 4-coumaric acid and rosmarinic acid, the sample volume was 10 μL, the flow rate was 1.0 mL/min, and the column temperature was 35°C. This method can realize the separation and determination of sodium danshensu, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, and 4-coumaric acid within 50 minutes. The linear relationships of the five peak areas and their concentrations are good (R2> 0.9997). The precision RSD values are all less than 1.0%. The reproducibility RSD values are all less than 1.3%. The stability RSD values are all less than 2.2%. The recovery values ranged from 92.4% to 99.4%. This method is simple, accurate, and reproducible. It can be used for the determination of sodium danshensu, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, and 4-coumaric acid in Danhong injection.
References
[1]
Zhang, J.X., Qi, M.J., Shi, M.Z., Chen, J.J., Zhang, X.Q. and Yang, J. (2019) Effects of Danhong Injection, a Traditional Chinese Medicine, on Nine Cytochrome P450 Isoforms in Vitro. Biomedical Chromatography, 33, e4454. https://doi.org/10.1002/bmc.4454
[2]
Li, W.Z., Xie, X.Y., Yang, J.Y., Zhao, F., Pan, J.Y. and Qu, H.B. (2022) NMR Method for Simultaneous Determination of 21 Chemical Components in Danhong Injection. China Journal of Chinese Materia Medica, 47, 6399-6408. https://doi.org/10.19540/j.cnki.cjcmm.20220419.301
[3]
Hao, L.J., Lang, A.H., Fan, C.Z. and Song, J.Y. (2020) Production and Quality Control of Original Herbal Materials of Danhong Injection. China Journal of Chinese Materia Medica, 45, 5443-5451. http://dx.doi.org/10.19540/j.cnki.cjcmm.20200915.302
[4]
Peng, S.X., Wei, C., Lei, J.Y., Zhang, T. and Ding, Y.B. (2023) Network Meta-Analysis of Chinese Medicine Injections for Activating Blood and Resolving Stasis in Adjuvant Treatment of Acute Ischemic Stroke. China Journal of Chinese Materia Medica, 48, 4215-4230. https://dx.doi.org/10.19540/j.cnki.cjcmm.20230425.501
[5]
Bi, C., Li, P.L., Zhang, W.J., Liu, H., Yi, J., Peng, W., et al. (2018) Correlation between Raw Herbs and Chemical Compounds in Danhong Injection. Central South Pharmacy, 16, 433-437. http://dx.doi.org/10.19540/j.cnki.cjcmm.20200915.302
[6]
Xu, L.L., Shang, Z.P., Bo, T., Sun, L., Guo, Q.L., Qiao, X. and Ye, M. (2019) Rapid Quantitation and Identification of the Chemical Constituents in Danhong Injection by Liquid Chromatography Coupled with Orbitrap Mass Spectrometry. Journal of Chromatography A, 1606, Article ID: 460378. https://doi.org/10.1016/j.chroma.2019.460378
[7]
Zuo, R., Xiao, M.J., Xu, D.P., Zheng, C.Y., Wang, W. and He, R.R. (2022) Salvianolic Acid a from Danhong Injection Induces Vasorelaxation by Regulating L-Type Calcium Channel in Isolated Mouse Arteries. Journal of Ethnopharmacology, 296, Article ID: 115431. https://doi.org/10.1016/j.jep.2022.115431
[8]
Li, Q.Q., Yuan, H.Y., Wang, L.T., Gan, X.H. and Qiao, C.D. (2011) Separation and Determination of Water-Soluble Active Components in Danhong Injection by Capillary Zone Electrophoresis. Journal of Lanzhou University (Medical Sciences), 37, 28-32. http://dx.doi.org/10.13885/j.issn.1000-2812.2011.04.003
[9]
Nijat, D., Xu, L., Kuang, Y., Yu, R., Zhang, Y. and Hasan, A. (2022) A Pharmacokinetic-Pharmacodynamic Study to Elucidate the Cardiovascular Protective Constituents in Danhong Injection. Journal of Pharmaceutical and Biomedical Analysis, 219, Article ID: 114953. https://doi.org/10.1016/j.jpba.2022.114953
[10]
Guan, X.Q., Mao, J.L., Tang, Y.X., Wang, J.H. and Sun, R. (2018) Research Progress on Pharmacological Effects of P-Coumaric Acid. Chinese Traditional and Herbal Drugs, 49, 4162-4170. http://dx.doi.org/10.7501/j.issn.0253-2670.2018.17.030
[11]
Yang, J., Liu, H.T., Wang, Y.F. and Zhu, Y. (2012) Study on the Stability of Multiple Bioactive Compounds in Danhong Injection by UPLC-UV Method. Tianjin Journal of Traditional Chinese Medicine, 29, 579-582.
[12]
Du, J.J., Qu, X.L. and Qi, Z. (2019) Determination of Components in Danhong Injection by HPLC. Orld Chinese Medicine, 14, 1373-1377. http://dx.doi.org/10.3969/j.issn.1673-7202.2019.06.006
[13]
Zhang, X.D., Cao, Z.X., Li, X.L., Lu, J., Song, Y.X. and Tang, J.F. (2015) Determination of Danshensu and Protocatechualdehyde in Danhong Injection by Ultra High Performance Liquid Chromatography. Acta Chinese Medicine, 4, 554-556. https://doi.org/10.16368/j.issn.1674-8999.2015.04.191
[14]
You, H.L., Wang, B. and Xu, H.L. (2012) Determination of Four Hydrosoluble Components in Danhong Injection by HPLC. Chinese Pharmaceutical Affairs, 26, 743-746. https://doi.org/10.3969/j.issn.1002-7777.2012.07.026
[15]
Li, G.S. and Hu, L.Z. (2015) Determination of 5 Water-Soluble Components in Danhong Injection by Hplc Method. Journal of North Pharmacy, 3, 1414-1415.
[16]
Gong, X.C., Huang, S.H., Jiao, R.N., Pan, J.Y., Li, Y. and Qu, H.B. (2016) The Determination of Dissociation Constants for Active Ingredients from Herbal Extracts Using a Liquid-Liquid Equilibrium Method. Fluid Phase Equilibria, 409, 447-57. https://doi.org/10.1016/j.fluid.2015.11.004
[17]
Pan, J.Y., Gong, X.C. and Qu, H.B. (2016) Degradation Kinetics and Mechanism of Lithospermic Acid under Low Oxygen Condition Using Quantitative H-1 NMR with Hplc-Ms. PLOS ONE, 11, e0164421. http://dx.doi.org/10.1371/journal.pone.0164421
[18]
Guo, Y.X., Zhang, D.J., Wang, H., Xiu, Z.L., Wang, L.X. and Xiao, H.B. (2007) Hydrolytic Kinetics of Lithospermic Acid B Extracted from Roots of Salvia Miltiorrhiza. Journal of Pharmaceutical and Biomedical Analysis, 43, 435-439. https://doi.org/10.1016/j.jpba.2006.07.046
[19]
Kan, S., Lin, H., Li, J., Shao, L. and Chen, D. (2012) Biotransformation of Salvianolic Acid B by Fusarium oxysporum f. sp. cucumerinum and Its Two Degradation Routes. Natural Product Communications, 7, 885-888.
[20]
Zhou, L.N., Zhang, X., Xu, W.Z., Ma, X.N., Jia, Z. and Zheng, Y.M. (2011) Studies on the Stability of Salvianolic Acid B as Potential Drug Material. Phytochemical Analysis: PCA, 22, 378-384. https://doi.org/10.1002/pca.1291
[21]
Lee, H.J., Cho, J.Y. and Moon, J.H. (2012) Chemical Conversions of Salvianolic Acid B by Decoction in Aqueous Solution. Fitoterapia, 83, 1196-1204. https://doi.org/10.1016/j.fitote.2012.06.015
[22]
Gong, X.C., Huang, S.C., Pan, J.Y. and Qu. H.B. (2017) Modeling of Degradation Kinetics of Salvianolic Acid B at Different Temperatures and Ph Values. Chinese Journal of Chemical Engineering, 25, 68-73. http://dx.doi.org/10.1016/j.cjche.2016.06.005
[23]
Li, Y., Guo, Z.-T., Gong, X.-C. and Qu, H.-B. (2013) Simultaneous Determination of Danshensu, Hydroxysafflor Yellow A, Rosmarinic Acid, Lithospermic Acid, Salvianolic Acid B in Water Extract of Mixed Salviae miltiorrhizae Radix et Rhizoma and Carthami flos by HPLC. China Journal of Chinese Materia Medica, 38, 1653-1656. http://dx.doi.org/10.4268/cjcmm20131101
