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光度法的五大贡献
The Five Major Contributions of Photometry

DOI: 10.12677/aac.2024.142016, PP. 131-140

Keywords: 分光光度法,“校准曲线”,消光强度,基态,光子流
Spectrophotometry
, “Calibration Curve”, Extinction Intensity, Ground State, Photon Flow

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Abstract:

以光度法样品测定的实例,展示了光度法的五大贡献:(1) 揭示了“校准曲线”蕴含的科学原理:“在信息量值域内,化学物质信息的改变量正比于其含量的改变量”,确立了“两标样测定法”,改变了传统观念;(2) 证明了“校准曲线”的含量上、下限,实际是其信息量的上、下限,任何含量的试样,只要制备成其信息量处于其信息值域内的样品,都可用其方法测定,拓宽了新方法的应用范围;(3) 鉴证了沉淀物悬浮液的选择性吸收、反射和散射效应,表明吸光度实际是选择性吸收、反射和散射的综合“消光强度”,完善了光度法的内涵,确立了“消光光度法”;(4) 证明了化学物质基态,实际是多种低能稳定态的“定比平衡分布”,提供了研究应用物质基态的新思路;(5) 证明了光线是不同于电磁波的光子流,为认识和研究光子提供了实验技术。
Actual sample measurements as an example, showcased the five major contributions of photometry: (1) it revealed the scientific principle contained in the “calibration curve”: “Within the range of information content, the change in chemical material information is proportional to the change in its content”, established the “two-standard sample determination method”, and changed traditional concepts; (2) it has been proven that the upper and lower limits of the “calibration curve” are actually the upper and lower limits of its information content. Any sample with any content, as long as it is prepared into a sample with its information content within its information value range, can be measured using its method, expanding the application range of the new method; (3) it verified the selective absorption, reflection, and scattering effects of sediment suspension, indicating that absorbance is actually the comprehensive “extinction intensity” of selective absorption, reflection, and scattering, improving the connotation of photometry and establishing the “extinction photometry”; (4) it has been proven that the ground state of chemical material is actually a “fixed proportion equilibrium distribution” of various low-energy stable states, providing a new ideas for studying and applying the ground state of material; (5) it proved that light is a photon flow different from electromagnetic waves, provided experimental techniques for understanding and studying photons.

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