Ultra-performance liquid chromatographic method for measurement of voriconazole in human plasma and oral fluid

a simple, sensitive and selective ultra-performance liquid chromatography method for the determination of voriconazole in plasma and oral fluid was developed and validated. after a liquid-liquid extraction with methyl-tert-butyl ether, the analyte and internal standard were separated on a hypersil gold c18 column (2.1 × 100 mm, p.d. 1.9 μm), eluted with a mobile phase composed of thietylammonium phosphate buffer and acetonitrile (70:30, v/v). total run time was 4 min, total mobile phase consumption of 2.2 ml. detection was performed with a photodiode array detector with quantitation at 256 nm. voriconazole concentrations in oral fluid were on average 57.5% (±5.3) of those measured in paired plasma samples.